Expression of stem cell surface marker CD133 in hepatocellular carcinoma and its positive subset proliferation
10.3969/j.issn.2095-4344.2017.09.002
- VernacularTitle:干细胞表面标志CD133在肝癌中的表达及其阳性亚群增殖特性
- Author:
Baoqin ZHANG
- From:
Chinese Journal of Tissue Engineering Research
2017;21(9):1319-1323
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND:CD133 has been used as a marker to separate and study different tumor stem cells by using its protein specific features. OBJECTIVE:To investigate the expression of stem cell surface marker CD133 in hepatocel ular carcinoma and the in vitro proliferation of its positive subsets. METHODS:The human hepatoma MHCC97-H cell lines were cultured and sorted, CD133+and CD133-MHCC97-H cells were obtained, and the expression of CD133 and cell migration and invasion were detected. After 7 days of culture, cell proliferation ability and Notch expression were detected. After 14 days of culture, cell cloning efficiency was detected. CD133+and CD133-MHCC97-H cells were implanted into nude mice subcutaneously, and 4 weeks later, tumor formation in mice was detected. RESULTS AND CONCLUSION:(1) CD133 expression and cell clone formation:The expression of CD133 and cell cloning efficiency of CD133+MHCC97-H cells were significantly higher than those of CD133-MHCC97-H cells (P<0.05). (2) Cell proliferation:After 3-7 days of culture, the absorbance value of CD133+MHCC97-H cells was significantly higher than that of CD133-MHCC97-H cells (P<0.05). (3) Cell migration and invasion:The number of CD133+MHCC97-H cells passing through the cell membrane was significantly more than that of CD133-MHCC97-H cells (P<0.05). (4) The Notch protein expression of CD133+MHCC97-H cells was significantly higher than that of CD133-MHCC97-H cells (P<0.05). (6) Tumor formation test:The tumor size of CD133 -MHCC97-H cells was larger than that of CD133-MHCC97-H cells (P<0.05). To conclude, CD133+hepatocel ular carcinoma cell subsets with strong proliferation ability have some characteristics of cancer stem cells, and strong invasion, metastasis and tumorigenic abilities.
