UPLC Fingerprint of the Root of Rosa Laevigata Michx from Different Regions
- VernacularTitle:不同产地金樱子根UPLC指纹图谱的研究
- Author:
Yulu LI
;
Guoxu MA
;
Jingquan YUAN
;
Wen ZHANG
;
Jieming ZOU
;
Xiaoqing ZHONG
;
Yanlin ZHOU
;
Zuolin SU
;
Nailiang ZHU
;
Xudong XU
;
Wei YI
- Keywords:
Roots of Rosa laevigata Michx;
UPLC;
Fingerprint;
Mutual mode
- From:
China Pharmacist
2017;20(3):409-412
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the fingerprint analysis method for the root of Rosa laevigata Michx from different regions by UPLC. Methods:The column was ACQUITY UPLC? Phenyl(2.1 mm × 100 mm,1.7 μm). The mobile phase consisted of methanol-water with gradient elution. The flow rate was 0. 2 ml·min-1 , the detection wavelength was 210 nm, the column temperature was 30℃, and the injection volume was 3 μl. Results:The fingerprint consisted of 15 common peaks. The range of similarity for twelve bat-ches of the root of R. laevigata Michx was 0. 489-0. 942. And the reference fingerprint of the root of R. laevigata Michx was estab-lished by UPLC. Conclusion:The fingerprint method is simple and reproducible, which can provide basis for the quality control and the medicinal resources exploration.
