Basic fibroblast growth factor-chitosan carrier induces neural stem cells to differentiate into neurons followed by co-culture with myocytes
10.3969/j.issn.2095-4344.2017.06.010
- VernacularTitle:碱性成纤维细胞生长因子-壳聚糖载体诱导神经干细胞分化及与肌细胞的共培养
- Author:
Jingyan ZHAI
;
Hongmei DUAN
;
Junkui SHANG
;
Zhaoyang YANG
;
Xiaoguang LI
- From:
Chinese Journal of Tissue Engineering Research
2017;21(6):877-882
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Neural stem cells (NSCs) hold self-renewal and multi-directional differentiation potential. NSCs differentiation into neurons in high proportion under induction conditions exhibits broad application prospect. OBJECTIVE:To explore the effect of basic fibroblast growth factor (bFGF)-chitosan carrier on the NSCs differentiation into neurons in vitro, and whether the differentiated neurons could form synaptic-like connection with myocytes. METHODS:After purification, the NSCs were co-cultured with chitosan, soluble bFGF or bFGF-chitosan carrier. After 7-day induction, the NSCs differentiation into neurons was observed by immunofluorescence staining of beta tubulin Ⅲ. The NSCs differentiation into cholinergic neurons was observed through double immunofluorescence staining of ChaT and beta tubulin Ⅲ. The synaptic-like connection between the neurons and myocytes was observed by triple staining of beta tubulin Ⅲ and MHC. RESULTS AND CONCLUSION:The percentage of differentiated neurons in the bFGF-chitosan carrier group was 74%, which was significantly higher than that in the other two groups. Additionally, the synaptic-like connection formed between the differentiated neurons and myocytes. To conclude, the bFGF-chitosan carrier promotes the NSCs differentiation into neurons to form synaptic-like connection with the co-cultured myocytes.
