Effect of different stimulants on the LAG3 expression and function of spleen lymphocytes in mice
10.3969/j.issn.1005-1678.2017.02.001
- VernacularTitle:不同刺激剂对小鼠脾脏淋巴细胞LAG3表达及功能的影响
- Author:
Nina XUE
;
Chunyang WANG
;
Yue CHEN
;
Dongjie WANG
;
Fangfang LAI
;
Xiaoguang CHEN
- Keywords:
lymphocytes;
lymphocyte activation gene 3;
interferon-γ;
cell proliferation
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(2):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different stimulants on the LAG3 expression and function of spleen lymphocytes in mice. Methods The spleen lymphocytes from mice were isolated by density centrifugation.The LAG3 expressions in T cell subsets after exposure to conA, PMA, PHA or anti-CD3/28 antibodies for 24 h or 72 h were analyzed by Flow cytometry.The IFN-γsecretions of conditional medium were detected by ELISA kit.The proliferation of lymphocytes was examined by MTT analysis.Results Treatment with conA for 24 h or 72 h dose-dependently increased LAG3 +CD3 +and LAG3 +CD4 +CD3 +T cell percentages.Similarly, an exposure of anti-CD3/28 antibodies for 72 h significantly increased LAG3 +CD3 +and LAG3 +CD4 +CD3 + T cell percentages.Meanwhile, conA and anti-CD3/28 antibodies increased the IFN-γsecretion of lymphocytes in a time-dependent manner.Furthermore, Treatment with conA, PMA, PHA or anti-CD3/28 antibodies for 72 h could enhance the proliferation of lymphocyte. Conclusion conA and anti-CD3/28 antibodies are effective activators of T cells, and both of them could promote the expression of LAG3 and IFN-γsecretion of lymphocytes.
