miR-498 inhibits A549 cell migration and invasion by down-regulating FOXM1
10.7652/jdyxb201702014
- VernacularTitle:miR-498通过下调FOXM1抑制肺腺癌细胞系A549的迁移侵袭
- Author:
Xi TANG
;
Ya HU
;
Yanhua XU
;
Chunlin WANG
;
Ping QIU
;
Xianghui WANG
- Keywords:
lung adenocarcinoma;
miR-498;
COL1A1;
COL1A5;
FOXM1;
invasion
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(2):226-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify whether miR-498 can inhibit A549 cell migration and invasion by down-regulating FOXM1.Methods miR-498 mimic and miR-NC were transfected into A549 cells.Wound healing and Transwell method were employed to test the migratory ability and invasion ability of A549 cells;Western blot was used to detect the expressions of COL1A1,COL1A5 and FOXM1 in A549 cells.Luciferase assay was used to confirm whether FOXM1 is the target gene of miR-498.Results Compared with those in the control group,the expressions of COL1A1,COL1A5 and FOXM1 were decreased,and the migration and invasion abilities of A549 cells were decreased in the miR-498 group (both P<0 .01 ).The luciferase activity of the FOXM1-3′-UTR plasmid was significantly suppressed by miR-498 (P<0 .05 );over-expression of FOXM1 could reverse the effect of miR-498 on A549 cells.Conclusion miR-498 inhibits A549 cell migration and invasion by down-regulating FOXM1.
