Clinical application of Carba NP test in detecting CRE strains

VernacularTitle:Carba NP试验检测碳青霉烯类耐药肠杆菌科细菌的临床应用
Author: Yuanting LI ; Fengling JIN
Keywords: carbapenemase; Carba NP test; enterobacteriaceae; drug-resistant gene
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2017;38(2):304-309
CountryChina
Language:Chinese
Abstract: Objective To investigate the clinical application of Carba NP test in detecting carbapenem-resistant enterobacteriaceae.Methods Routine method was adopted to identify the 51 strains from clinical isolates and K-B test was conducted to screen out the carbapenem-resistant enterobacteriaceae and detect antimicrobial susceptibility.E-test was used to detect the minimum inhibitory concentration (MIC)of carbapenems.Modified Hodge test (MHT)and Carba NPⅠ test were used to screen out the phenotypes of carbapenemase,respectively. Then Carba NPⅡ test was used to classify carbapenemase which were positive in Carba NP I test.E-test was used for the detection of metalloenzymes.Polymerase chain reaction (PCR)was used for the detection of resistance genes. Results Totally 41 strains were positive in Carba NP 1 test and classified as B-class by Carba NPⅡ test.They were positive in metalloenzyme by E-test and carried NDM-1 gene by PCR.Conclusion Carba NP test can be used conveniently,has high consistency with PCR method,and the result is easy to determine.Therefore,it can be used as a routine laboratory method.