Effects of exogenous hydrogen sulfide on the expressions of angiotensinⅡand proliferating cell nuclear an-tigen of tubulointerstitial fibrosis in rats with unilateral ureteral obstruction
10.16571/j.cnki.1008-8199.2017.02.008
- VernacularTitle:外源性硫化氢对大鼠梗阻肾组织中血管紧张素Ⅱ和PCNA及TGF-β1表达的影响
- Author:
Dean ZHAO
;
Qian HUANG
;
Jun LIU
;
Ziming HAN
- Keywords:
Hydrogen sulfide;
Ureteral obstruction;
Tubulointerstitial fibrosis;
Proliferating cell nuclear antigen;
AngiotensinⅡ;
Transforming growth factor-beta
- From:
Journal of Medical Postgraduates
2017;30(2):149-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective Tubulointerstitial fibrosis(TIF) is the most important marker reflecting the degree of renal function decline and prognosis and hydrogen sulfide ( H2 S) is crucial in maintaining normal renal function and many diseases of renal injury. The aim of the article was to investigate the effects of exogenous H2 S on the expressions of angiotensinⅡ ( AngⅡ) , proliferating cell nuclear antigen (PCNA) and transforming growth factor beta-1 (TGF-β1) in rats with unilateral ureteral obstruction (UUO). Methods TIF rat model was built with UUO. Ninety-six SD rats were randomly divided into four groups:sham operation group, modelgroup, UUO+low-dose NaHS treatment group ( low dose group) and UUO+high-dose NaHS treatment group ( high dose group) ( n=24, respectively) . Rats in model group were treated with left-side ureteral obstruction and ureteral separation without obstruction was done in sham operation group. UUO rats in two treatment groups were injected intraperitoneally with two different doses of sodium hydrosulfide (NaHS, donor of endogenous H2 S), respectively. HE and Massonstaining and immunohistochemical staining were performed at the 7 d, 14 d and 21 d, respectively. Results In sham operation group, the expressions of AngⅡ, PCNA, and TGF-β1 were found in microamount in tubulointerstitium at each time points. Compared with sham operation group, the expressions of AngⅡ, PCNA and TGF-β1 in model group increased( P<0.01) . While in comparison to model group, the expressions of AngⅡ, PCNA and TGF-β1 decreased in low dose group and high dose group, but no significant differ-ence was found between low dose group and high dose group. Conclusion Exogenous H2 S supplementation can attenuate TIF partly via downregulating the expressions of AngⅡ, PCNA and TGF-β1 .
