Effects of denatonium benzoate on calcium binding protein S100A9 and mucus in the airways of asthmatic mice

VernacularTitle:苦精对哮喘小鼠气道中钙结合蛋白S100A9及气道黏液分泌的影响
Author: Shan YAN ; Li CHENG ; Xiaoning ZHAO ; Yimin HUANG ; Jingjing LIU ; Fusheng GAO
Keywords: Denatonium benzoate; Asthma; Bitter taste receptor; Ca2+-binding protein S100A9; Airway mucus
From: Journal of Medical Postgraduates 2017;30(2):155-159
CountryChina
Language:Chinese
Abstract: number of goblet cells, mucus secretion and mucin MUC5AC content in lung tissues. Results S100A9 in BALF of group B was (11.89±0.77) ng/mL, S100A9 integrated optical density (IOD) value in airway epithelial cells was 13.96±1.62, PAS stain area /epithelial cell area was (12.53±1.21)%, relative value of MUC5AC / NADPH was 173.91±4.29, all of the above were higher than those of group A [(6.19±0.61) ng/mL, 4.97±0.30, (1.94±0.18)%, 1];S100A9 levels, IOD of S100A9 in airway epithelial cells, PAS stain area / epithelial cell area (%), relative value of MUC5AC / NADPH in group C [(10.69±0.79) ng / ml, 11.80±0.72, (10.61±0.61)%, 94.65±1.59], group D[(9.49±0.99) ng/mL, 10.39±0.59, (8.63±0.62)%, 82.08±1.12], group E [(7.54± 0.42) ng/mL, 5.63±0.84, (4.59±0.87)%, 26.30±1.94] were lower than group B, which showed a dose-dependent reduction and the difference was statistically significant (P<0.05 or P<0.01). Conclusion DB downregulates the expression level of Ca2+-binding protein S100A9 and the mucus secretion amount of the airway goblet cells in rats.