Effects of IL-18 gene combined with diterpenoid alkaloids in inducing proliferation and promoting apoptosis of tongue squamous carcinoma cells
10.7652/jdyxb201702012
- VernacularTitle:IL-18基因联合二萜生物碱对舌鳞癌细胞的增殖抑制与促凋亡作用
- Author:
Yalin HU
;
Meiling HOU
;
Wenjiao ZHENG
;
Zenan ZHANG
;
Shaoheng HE
;
Zhigang LI
- Keywords:
interleukin-18 (IL-18 );
diterpenoid alkaloid;
combination therapy;
squamous cell carcinoma;
inhibition of proliferation;
apoptosis
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(2):215-220
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of human IL-18 gene combined with diterpenoid alkaloids in inhibiting the proliferation and inducing the apoptosis of tongue squamous carcinoma cells Tscca.Methods We constructed recombinant plasmid pEGFPN3-IL-18 and tranfected it into tongue squamous carcinoma cells Tscca.The transduction efficiency of the target cells was detected by fluorescent microscopy,cytotoxic effect of IL-18 gene with diterpenoid alkaloids on Tscca was detected by MTT assay,and apoptosis was detected by flow cytometry. Western blot was employed to examine the expression level of cellular signal-regulated kinase Akt/p-Akt.Results The tongue squamous cells Tscca which transfected pEGFPN3-IL-18 had significantly increased apoptosis compared with non-transfected cells (P<0 .05 ).Tongue carcinoma squamous cells cultured with diterpenoid alkaloids at the concentrations of 0 .2 ,0 .4 and 0 .6 mg/mL had significantly increased apoptosis in a dose-dependent manner (P<0.05).Human IL-18 gene combined with diterpenoid alkaloids for 48 hours inhibited significantly Tscca in a concentration-dependent manner compared with diterpenoid alkaloids alone (P<0 .05 ).The two in combination could also decrease the protein level of p-Akt dose-dependently.Conclusion The combination of pEGFPN3-IL-18 and diterpenoid alkaloids has a synergistic effect in inhibiting the growth of tongue squamous carcinoma cells Tscca.
