Inhibitory effect of curcumin on Aβ1-42-induced cell damage and mitochondrial pathway apoptosis
10.3867/j.issn.1000-3002.2017.02.03
- VernacularTitle:姜黄素对Aβ1-42诱导的细胞损伤和线粒体途径细胞凋亡的抑制作用
- Author:
Shuyan LU
;
Li YANG
;
Xueling DAI
;
Ping CHANG
;
Zhaofeng JIANG
;
Hanchang HUANG
- Keywords:
Alzheimer disease;
curcumin;
β amyloid protein;
mitochondrial membrane potential;
apoptosis
- From:
Chinese Journal of Pharmacology and Toxicology
2017;31(2):138-144
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the protective effect of curcumin on Aβ1-42 damaged cells. METHODS SH-SY5Y cells were cultured with Aβ1-4210μmol · L-1 in the absence or presence of curcumin 1, 5 or 10 μmol · L-1. Cell viability was assayed by MTT. Cell membrane damage was detected by the concentration of lactate dehydrogenase (LDH) in culture medium. Cell apoptosis was measured by flow cytometry with Annexin Ⅴ-FITC/PI staining. Mitochondrial membrane potential was characterized by fluorescence of JC-1 dye. Enzymatic activity of caspases-9 and-3 was measured by colorimetric assay. Protein expression of caspase-3 was detected by Western blotting. RESULTS Compared with vehicle control, the cell viability, concentration of LDH and both early and late apoptosis in Aβ1-4210 μmol · L-1 damaged group were decreased(P<0.01). However, the cell viability, release of LDH and both early and late apoptosis in curcumin group were promoted compared with that in Aβ1-4210μmol·L-1 damaged group. Curcumin inhibited Aβ1-42-induced depolarization of mitochondrial membrane potential(P<0.01), and attenuated Aβ1-42-induced activation of both caspases9 and caspases3 in a concentration-dependent manner, respectively(r=0.990, P<0.01; r=0.996, P<0.01). There were no significant differences in the above detected indexes between curcumin 10 μmol · L-1 group and vehicle control group. CONCLUSION Curcumin inhibits Aβ1-42-induced cell damage and apoptosis by promoting mitochondrial membrane potential and depressing the activation of caspases.
