Effects of JNK inhibition on insulin signaling pathway in HepG2 cells
10.3760/cma.j.issn.1000-6699.2017.02.008
- VernacularTitle:抑制JNK活性对HepG2细胞胰岛素信号通路的影响
- Author:
Kaixuan LI
;
Xiaolin JI
;
Cheng SUN
;
Lan LUO
- Keywords:
JNK;
JNK inhibitors;
MAPK signaling pathway;
Insulin signaling pathway;
HepG2 hepatocytes
- From:
Chinese Journal of Endocrinology and Metabolism
2017;33(2):129-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of JNK inhibition on insulin signaling pathway. Methods HepG2 cells were treated with different kinds of JNK inhibitors for 12 h, and then the cells were treated with 10 nmol/L insulin for 5 min to stimulate insulin signaling pathway. Mitogen-activated protein kinases ( MAPK ) and insulin signaling pathways were analyzed by Western blot using the total cell lysates. Results JNK activity was significantly inhibited by JNK inhibitor JNKi-Ⅷand results showed that JNKi-Ⅷtreatment could reduce insulin signaling pathway in a dose-dependent manner. Furthermore, other JNK inhibitors including JNKi-Ⅴ, JNKi-Ⅲ, and SP600125 blocked JNK activity in HepG2 cells. Similar to JNKi-Ⅷ, these JNK inhibitors also impaired insulin signaling transduction in a dose-dependent manner. Conclusion In HepG2 hepatocytes, JNK activity inhibition blocks insulin signaling transduction.
