Application of PCR-fluorescent probe in identifying genotypes of HCV
10.3760/cma.j.issn.1674-2397.2017.01.008
- VernacularTitle:PCR-荧光探针法在中国HCV常见基因型检测中的意义
- Author:
Tao WU
;
Huafang LIANG
;
Lu XIONG
;
Jiao WANG
;
Xiaolei GUO
;
Feng LIN
- Keywords:
Hepatitis C;
Genotype;
Polymerase chain reaction;
PCR-fluorescence probe method;
Nested reverse transcription -PCR;
Phylogenetic tree
- From:
Chinese Journal of Clinical Infectious Diseases
2017;10(1):37-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the application value of polymerase chain reaction (PCR)-fluorescence probe method in identifying genotypes of hepatitis C virus (HCV).Methods One hundred and sixty six serum samples from patients with chronic HCV infection were collected nationwide from March to June 2016.HCV Core-E1 gene region was amplified and sequenced by nested reverse transcription-PCR (RT nested-PCR)and genetic subtypes were analyzed by phylogenetic tree,meanwhile HCV genotypes were also determined by PCR-fluorescent probe method.Kappa test was used to compare the consistency of two methods.Results Among 166 samples detected by RT nested-PCR,the genotype of 66 samples (39.8%) was 1 b,34 (20.5%)was 2a,16 (9.6%)was 3a,27 was 3b (16.2%),23 (13.9%)was 6a.Two samples with 3b genotype detected by RT nested-PCR were identified as 1 b by PCR-fluorescent probe.The consistency rate of two methods was 98.7% (164 /166),there was no significant difference between two methods (χ2 =0.0492,P >0.05).Conclusion PCR-fluorescence probe method can accurately identify HCV genotypes and can be used in clinic.
