A Dual-signal Amplification Method for DNA Detection Based on Exonuclease Ⅲ and Fluorescence Quenching Ability of MoS2 Nanosheet
10.11895/j.issn.0253-3820.160642
- VernacularTitle:基于核酸外切酶Ⅲ诱导的双重信号放大与MoS2纳米片荧光碎灭性质的核酸检测方法
- Author:
Yufei LIU
;
Jintao XUE
;
Huijuan YAN
;
Lijuan YANG
;
Wei LIU
;
Xiangde SUN
- Keywords:
DNA sensor;
Molybdenum disulfide nanosheet;
Exonuclease Ⅲ;
Dual-signal amplification method;
Quenching ability
- From:
Chinese Journal of Analytical Chemistry
2017;45(3):303-308
- CountryChina
- Language:Chinese
-
Abstract:
A highly sensitive and selective DNA biosensor is described based on the fluorescence quenching ability of MoS2 nanosheet and exonucleaseⅢ( ExoⅢ) assisted dual-signal amplification. In this sensor, the fluorescence probes ( HP1 and HP2 ) cannot be degraded by Exo Ⅲdue to the 3 '-termini protrusion and thus are adsorbed on the surface of MoS2 nanosheets, which will result in the quenching of MoS2 nanosheets toward the probes and induce a low fluorescent signal. The presence of the target DNA leads to the desorption of probes on the surface of MoS2 nanosheets due to the hybridization toward probes, generating many fluorescent fragments by Exo Ⅲdigestion and inducing dual-signal amplification. This method can improve the sensitivity and detection limit compared with single amplification method, and shows excellent selectivity in the discrimination of single base mismatched targets. On the basis of the significantly high sensitivity, the developed biosensor can be potentially extended to detect various DNA targets with excellent sequence selectivity.
