An Untargeted Urinary Metabolomics Strategy for Investigation of Therapeutical Mechanism of Schisandra chinensis on Complications of Diabetes Rats
10.11895/j.issn.0253-3820.160753
- VernacularTitle:非靶向尿液代谢组学方法研究五味子治疗精尿病并发症作用机制
- Author:
Hongxiang HAN
;
Fengrui SONG
;
Zhenbo SHU
;
Zhiqiang LIU
;
Yueying REN
;
Zifeng PI
- Keywords:
Untargeted metabolomics;
Schisandra chinensis;
Diabetes mellitus and its complications;
Mechanism
- From:
Chinese Journal of Analytical Chemistry
2017;45(3):389-396
- CountryChina
- Language:Chinese
-
Abstract:
An untargeted urinary metabonomics method based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry ( UPLC-Q-TOF-MS ) has been established to investigate the mechanism of Schisandra chinensis in treating diabetes and its complications. The urinary biomarkers related to the therapeutic effects of Schisandra chinensis on the diabetes rats were analyzed. In urine, 28 kinds of endogenous metabolites were identified as potential biomarkers, including 13 endogenous metabolites in positive ion mode, 15 endogenous metabolites in negative ion mode, and hippuric acid detected both in positive and negative ion modes. The results revealed that Schisandra chinensis mainly affected the pathways of pentose and glucuronate interconversions, riboflavin metabolism, pantothenate and CoA biosynthesis, arginine and proline metabolism, intestinal bacteria metabolism, ascorbate and aldarate metabolism and tryptophan metabolism in diabetic rats. Combined with biological analysis of these pathways, the therapeutic mechanism of Schisandra chinensis on diabetes and its complications was verified. Based on the biological function of each pathway, the effect of Schisandra chinensis on diabetic nephropathy is stronger. Moreover, it also has the effects of protecting liver, decreasing fat and antioxidant activity.
