Determination of catechin in Songling-Xuemaikang capsules by HPLC
10.3760/cma.j.issn.1673-4246.2017.03.015
- VernacularTitle:高效液相色谱法测定松龄血脉康胶囊中儿茶素含量
- Author:
Yingjiao ZHANG
;
Jia WU
;
Jian CHEN
;
Chen XIA
;
Junlin DENG
- Keywords:
Catechin;
Chromatography;
high pressure liquid;
Songling-Xuemaikang capsules;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2017;39(3):250-253
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determination of the content of catechin in Songling- Xuemaikang capsules. Methods The analysis was performed on Alltima C18 column (250 mm × 4.6 mm, 5 μm) with mobile phase consisting of methanol (A) -l% Acetic Acid (B) for gradient elution. The flow rate was 1 ml/min and the column temperature was 25 ℃. Results The results showed that catechin was well separated with the good linearity in 0.00756-0.05040 μg/μl. The average recovery of catechin was 100.147%. Conclusions The method could be applied to determine catechin in Songling-Xuemaikang capsules, which would make sure the quality control of capsules.