Determination of 8 Kinds of Cephalosporins in Aquatic Products by Multi-walled Carbon Nanotubes Solid Phase Extraction-Ultra High Performance Liquid Chromatography-Mass Spectrometry
10.11895/j.issn.0253-3820.160633
- VernacularTitle:多壁碳纳米管净化-超高效液相色谱-质谱法测定水产品中头孢菌素残留量
- Author:
Beiqiao GU
;
Guangming MEI
;
Xiaojun ZHANG
;
Yina HE
;
Zhongyong YAN
;
Jingping ZHU
- Keywords:
Multi-walled carbon nanotubes;
Ultra-high performance liquid chromatography-mass spectrometry;
Aquatic product;
Cephalosporins
- From:
Chinese Journal of Analytical Chemistry
2017;45(3):381-388
- CountryChina
- Language:Chinese
-
Abstract:
An ultra-high performance liquid chromatography-mass spectrometry ( UPLC-MS/MS) method was developed for the determination of 8 kinds of cephalosporins, cefoperazone, cefquinome, cefalonium, cefazolin, cefapirin, Ceftiofur, cefpirome and cefalexin, in edible parts of aquatic products. The samples were extracted with acetonitrile-water and cleaned up by multi-walled carbon nanotubes ( MwCNTs) SPE cartridge. All the target compounds were separated on an Acquity Xselect CSH C18 column with gradient elution by using acetonitrile and 0. 1% formic acid aqueous as eluent, and detected by UPLC-MS/MS under ESI+ ionization and MRM mode. Under optimized conditions, this method had a good linearity (R2≥0. 995) and the limits of quantification were in the range of 2-10 μg/kg ( S/N=10 ) . The recoveries of the method for the target compounds spiked at three different levels were 67. 3%-94. 2% with the relative standard deviations (RSDs) of 3. 3%-14%. The method had the characteristics of low cost, high accuracy and good precision, and could meet the requirements of cephalosporins determination.
