Development of an Enhanced Colloidal Gold Immunochromatographic Assay for Detection of Imidaclothiz
10.11895/j.issn.0253-3820.160730
- VernacularTitle:氯噻啉胶体金增强免疫层析分析方法的建立
- Author:
Haiyan SHI
;
Enze SHENG
;
Ming MA
;
Xiude HUA
;
Minghua WANG
- Keywords:
Imidaclothiz;
Monoclonal antibody;
Gold immunochromatographic assay;
Streptavidin;
Biotin
- From:
Chinese Journal of Analytical Chemistry
2017;45(3):403-408
- CountryChina
- Language:Chinese
-
Abstract:
An enhanced gold immunochromatographic assay ( GICA ) with simplicity, rapidity and high sensitivity was developed to detect imidaclothiz by using the high affinity between biotin and streptavidin. The 13-nm AuNPs were double-labeled with anti-imidaclothiz antibody and biotinylated DNA, and the 41-nm AuNPs were labeled with streptavidin to prepare an enhanced gold immunochromatographic test strip for imidaclothiz. The working conditions of the strip were systematically optimized, and the sensitivity, specificity, precision and accuracy were assessed by testing the cross-reactivity ( CR) , spiked recovery and validation with high performance liquid chromatography ( HPLC) . Under the optimal conditions, the detection could be completed in 10 min with visual result, and the limit of detection ( LOD ) was 25 ng/mL. The analysis showed no cross-reactivity with analogues of imidaclothiz except for imidacloprid. The detection results of GICA agreed with the spiked concentrations of imidaclothiz at spiked levels of 0 . 05 , 0 . 5 and 5 μg/g in river water, rice, cucumber, tomato, pear, cabbage and apple samples. The detection results of GICA for imidaclothiz in unknown concentration river water and pear samples were consistent with that of HPLC.
