Monosaccharide Composition Analysis of Arabinoxylan by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection
10.11895/j.issn.0253-3820.160685
- VernacularTitle:基于高效阴离子色谱-脉冲安培法优化阿拉伯木聚精单精组成分析
- Author:
Xiaoying LIU
;
Luyao MA
;
Li WANG
;
Xingyi WANG
;
Shaoping NIE
;
Mingyong XIE
;
Junyi YIN
- Keywords:
Arabinoxylan;
Seeds from Plantago asiatica L.;
High performance anion exchange chromatography;
Hydrolysis condition;
Monosaccharide composition
- From:
Chinese Journal of Analytical Chemistry
2017;45(3):416-422
- CountryChina
- Language:Chinese
-
Abstract:
A method of complete acid hydrolysis combined with high performance anion exchange chromatography and pulsed amperometric detection was developed for the monosaccharide composition analysis of arabinoxylan from the seeds of Plantago asiatica L. The parameters including hydrolysis methods, acid types, acid concentration, hydrolysis temperature, hydrolysis time and placement time, which would affect the hydrolysis process, were optimized. The results showed that it would have a better hydrolysis effect for polysaccharide from the seeds of Plantago asiatica L. with 2 mol/L H2 SO4 in an atmospheric oil bath at 120℃for 2 hours. However, the placement time for diluted solution of the hydrolyzed polysaccharide should be less than 6 hours. The polysaccharide was mainly composed of Arabinose (8. 89%) and Xylose (41. 52%) and Galacturonic acid (0. 73%). Glcuronic acid (3. 44%) was detected simultaneously, and there were also trace amounts of Galatose and Glucose. The results were reproducible. Other arabinoxylans from Panicummiliaceum L. shell, Avena sativa L. bran and Hordeum vulgare L. were taken for monosaccharide compositions analysis under the optimal hydrolysis conditions and the analysis results were good. This study would provide a good reference for monosaccharides composition analysis of arabinoxylans from various sources.
