Multi-target Determination of Trace Endocrine Disrupting Chemicals in Biota Using Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.160651
- VernacularTitle:超高效液相色谱-串联质谱多目标分析生物样品中的微量内分泌干扰污染物
- Author:
Jun LIU
;
Caiming TANG
;
Songsong XIONG
;
Yujuan FAN
;
Ke ZHENG
;
Xianzhi PENG
- Keywords:
Endocrine disrupting chemicals;
Ultra-high performance liquid chromatography-tandem mass spectrometry;
Biota
- From:
Chinese Journal of Analytical Chemistry
2017;45(3):434-440
- CountryChina
- Language:Chinese
-
Abstract:
An ultra-high performance liquid chromatography-tandem mass spectrometry ( UHPLC-MS/MS ) method was developed and validated for the simultaneous determination of 9 kinds of trace endocrine disrupting chemicals in biological samples using ultrasonic-assisted extraction followed by purification with gel permeation chromatography ( GPC) and silica gel columns. The sample extracts were purified by Bio beads S-X3 GPC columns with cyclohexane/ethyl acetate (1:1, V/V) as mobile phase, and the target compounds were eluted in the fraction of 12-28 mL retention volume. Electrospray ionization source operated in positive mode and atmospheric pressure chemical ionization source operated in negative mode were used for mass spectrometric detection. Data acquisition was carried out in multiple reaction monitoring mode. Recoveries were predominately within 65 . 2%-118 . 0%. Method quantification limits were 0 . 1-9 . 7 ng/g dw ( dry weight ) . This method was successfully applied to the analysis of the target endocrine disrupting chemicals in carps collected from the Pearl River. with the exception of carbanilide and triclocarban, the rest analytes were detected in fish tissue samples, with the concentrations varied within the range of 0. 1-22. 6 ng/g dw.
