Study on cyfluthrin resistance and its mechanisms of Anopheles sinensis in Nanchang frontierport
10.16250/j.32.1374.2016200
- VernacularTitle:南昌国境口岸中华按蚊氟氯氰菊酯抗药性及其机制研究
- Author:
Tielong XU
;
Qiang ZHANG
;
Lan LIU
;
Minghui ZHAO
;
Yun LIAO
;
Lixin LIAO
;
Jianjun WANG
;
Bin ZHENG
- Keywords:
Anopheles sinensis;
Cyfluthrin;
Resistance;
P450 monooxygenase;
Glutathione S-transferase;
Changbei Inter-national Airport
- From:
Chinese Journal of Schistosomiasis Control
2017;29(2):146-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the cyfluthrin resistance and potential mechanisms of Anopheles sinensis in Nanchang Chang-bei International Airport,Nanchang City,Jiangxi Province. Methods The resistance levels of the local An. sinensis were de-tected by WHO drug resistance bioassay. During the bioassay,the dying mosquitos were classed as sensitive mosquitos,and the survival ones were classed as resistant mosquitos. The P450 monooxygenase activity and glutathione S-transferase activity were detected and compared between the two groups. At the same time,the death time of each sensitive mosquito was recorded,and the correlations between the death time and the P450 monooxygenase activity and glutathione S-transferase activity were ana-lyzed,respectively. Results The bioassay mortality of the local An. sinensis was 59.5%. The differences of the P450 monooxy-genase activities among the resistant mosquitos,sensitive mosquitos and laboratory sensitive mosquitos had statistical signifi-cances(F=151.89,P<0.01),the resistant mosquitos>sensitive mosquitos>laboratory sensitive mosquitos. The differences of glutathione s-transferase activities among the three groups had no statistical significance(F=0.72,P=0.49). There existed positive correlation between the mosquito death time and the P450 monooxygenase activity,and the regression equation was y=79.479+1.512x with the correlation coefficient of 0.88,while there was no correlation between the mosquito death time and the glutathione S-transferaseactivity. Conclusion The An. sinensis in Nanchang Changbei International Airport has been resistant to cyfluthrin,and the promotion of P450 monooxygenase activity maybe one of the reasons for the resistance.
