Effect of Parecoxib Sodium Pretreatment on Permeability of Blood-Brain Barrier in a Rat Model of Focal Cerebra1 Ischemia-reperfusion Injury
- VernacularTitle:帕瑞昔布钠预先给药对大鼠局灶脑缺血再灌注血脑屏障通透性的影响
- Author:
Shaoxing LIU
;
Xianfeng XIE
;
Dejun CAO
;
Mengchang YANG
;
Yuee DAI
- Keywords:
Parecoxib;
Cerebral ischemia reperfusion injury;
Blood-brain barrier;
Cytokines
- From:
Journal of Kunming Medical University
2016;37(8):47-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and the mechanism of parecoxib sodium pretreatment on permeability of blood-brain barrier in a rat model of focal cerebral ischemia-reperfusion injury.Methods Sixty male SD rats weighing 300g were randomly divided into 5 groups (n=12 each):sham operation group (group S);focal cerebral I/R group (group I/R);parecoxib sodium 5 mg/kg pretreatment group (group L);parecoxib sodium7.5mg/kg pretreatment group (group M);parecoxib sodium 10 mg/kg pretreatment group (group H) Middle cerebral artery occlusion models were made by reforming Longa suture method in SD rats.Thirty minutes before ischemia,rats in group L,M and H were injected with 5 mg/kg、7.5 mg/kg and 10 mg/kg parecoxib sodium through the internal jugular vein.Group S and group I/R received equal volume of normal saline.ELISA technique was used to determine the content of S100 β,TNF-α,IL-1 β in Plasma.The changes of cerebral water content and the Evans Blue exudation from brain capillaries were observed.Results Pretreated with parecoxib sodium (5mg/kg、7.5 mg/kg and 10 mg/kg),the content of S100 β,TNF-α,II-1 β in plasma were reduced.The cerebral water content and the EB in brain were reduced.Pretreated with parecoxib sodium 10 mg/kg,Longa scores were reduced.Conclusion Pretreatment with Parecoxib can protect blood-brain barrier against focal cerebral I/R injury by inhibition of the inflammatory reaetion.
