Content Determination of Cabozantinib in Its Raw Material by RP-HPLC
10.6039/j.issn.1001-0408.2017.12.31
- VernacularTitle:RP-HPLC法测定卡博替尼原料药中卡博替尼的含量
- Author:
Wei XU
;
Changjuan ZHAN
;
Hua WANG
;
Yi WANG
;
Qi GUO
- Keywords:
Cabozantinib;
RP-HPLC;
Content determination
- From:
China Pharmacy
2017;28(12):1696-1698
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of cabozantinib in its raw material. METHODS:RP-HPLC method was adopted. The determination was performed on Inertsil ODS-SP C18 column with mobile phase consisted of acetonitrile-0.02 mol/L ammonium acetate buffer (pH 5.2,52:48,V/V) at the flow rate of 1.0 mL/min. Detection wavelength was set at 241 nm,the column temperature was 38 ℃,and sample size was 20 μL. RESULTS:The linear range of cabozantinib were 9.88-49.40 μg/mL(r=0.9999). The limit of quantitation was 11.46 ng,and the limit of detection was 3.36 ng. The RSDs of preci-sion,stability,repeatability tests were all lower than 2.0%;recoveries were 98.5%-101.7%(RSD=1.2%,n=9). CONCLU-SIONS:The method is simple,accurate and suitable for the content determination of cabozantinib in its raw material.
