Simultaneous Determination of 9 Common Inorganic Anions in Huanglian Shangqing Tables by Accelerat-ed Solvent Extraction-ion Chromatography

VernacularTitle:加速溶剂萃取-离子色谱法同时测定黄连上清片中9种常见无机阴离子的含量
Author: Enze HU ; Ming WEI ; Zhenghui HUANG
Keywords: Accelerated solvent extraction; Ion chromatography; Huanglian shangqing tablet; Inorganic anion
From: China Pharmacy 2017;28(12):1706-1710
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To establish the method for simultaneous determination of 9 common inorganic anions in Huanglian shangqing tablet. METHODS:The accelerated solvent extraction-ion chromatography was adopted. Inorganic anions were deter-mined by Ion Pac AS11-HC anion exchange column,protected by Ion Pac AG11-HC column and eluted by hydroxide potassium so-lution(gradient elution)at the flow rate of 1.2 mL/min. The column temperature was set at 30 ℃,elution time was 18 min,and sample volume was 25 mL. RESULTS:The linear ranges of fluorinion,formate ion,nitrite ion,bromide ion,nitrate ion,sulfate ion,oxalate ion and phosphate ion were 0.1-5 mg/L(r=0.9990-0.9999). The limits of quantitation were 0.020,0.078,0.030, 0.058,0.052,0.068,0.084,0.064,0.074 mg/L,and the limits of detection were 0.005,0.024,0.008,0.017,0.015,0.022, 0.026,0.020,0.021 mg/L,respectively. RSDs of precision,stability and repeatability were all lower than 4.0%;recoveries were 80.00%-125.08%(RSD ranged 0.97%-2.47%). CONCLUSIONS:The method is simple,precise,stable and repeatable,and can be used for 9 common inorganic anions in Huanglian shangqing tablet.