Effect of hydrogen on mitochondrial dynamics during endotoxin-induced damage to human umbilical vein endothelial cells
10.3760/cma.j.issn.0254-1416.2017.02.026
- VernacularTitle:氢对内毒素诱导人脐静脉内皮细胞损伤时线粒体动力学的影响
- Author:
Yanyan WANG
;
Aili DONG
;
Yuanyuan ZHANG
;
Lu WANG
;
Yingxue BIAN
;
Hongguang CHEN
;
Guolin WANG
;
Yonghao YU
;
Keliang XIE
- Keywords:
Hydrogen;
Endotoxemia;
Blood vessels;
Endothelial cells;
Mitochondria
- From:
Chinese Journal of Anesthesiology
2017;37(2):227-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of hydrogen on mitochondrial dynamics during endotoxin-induced damage to human umbilical vein endothelial cells (HUVECs).Methods HUVECs cultured in vitro were seeded in the culture plate and divided into 4 groups using a random number table:control group (group C),hydrogen-saturated culture medium group (H group),endotoxin group (group E) and endotoxin + hydrogen-saturated culture medium group (group E+H).The cells were cultured in the plain culture medium in C and E groups.The cells were cultured in the hydrogen-saturated culture medium containing lipopolysaccharide (LPS) with the final concentration of 10 μg/ml in H and E+H groups.At 2,8 and 24 h of culture or incubation with LPS,the cell viability was detected by methyl thiazolyl tetrazolium assay,the intracellular ATP content was measured using the phosphomolybdic acid colorimetric method,and the expression of dynamin-related protein 1 (DRP1) was detected by using Western blot.The expression of DRP1 was detected by immunofluorescence at 8 h of incubation with LPS.Results Compared with group C,the cell viability and ATP content were significantly decreased,and the expression of DRP1 was up-regulated at each incubation time point in E and E +H groups (P＜0.05),and no significant change was found in the parameters mentioned above in group H (P＞0.05).Compared with group E,the cell viability and ATP content were significantly increased,and the expression of DRP1 was down-regulated at each incubation time point in group E+H (P＜0.05).Conclusion The mechanism by which hydrogen reduces endotoxin-induced damage to HUVECs is related to down-regulation of DRP1 expression and inhibition of excessive mitochondrial fission.
