Effects of Zhen Gan Xi Feng (ZGXF) Decoction on Amphetamine-Induced Rotation in Rats with Parkinson's Disease (PD) with the Syndrome of Liver-Yang Hyperactivity
10.11842/wst.2016.12.019
- VernacularTitle:镇肝熄风汤对帕金森病肝阳上亢证大鼠旋转行为的影响
- Author:
Xiaoming LI
;
Lihua ZHANG
;
Yanqiu QI
;
Ying SUN
;
Lanqin ZHU
;
Miaoxian DONG
- Keywords:
Zhen Gan Xi Feng decoction;
rotation behavior;
transcription factor NF-e2-related factor 2;
syndrome of liver-yang hyperactivity;
Parkinson's disease
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2016;18(12):2137-2142
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to explore the effects of ZGXF decoction on amphetamine-induced rotation in PD rats with syndrome of liver-yang hyperactivity,and its mechanism involved.Rats received 6-OHDA administration via intra-substantia nigra injection and were intragastrically treated by Fu Zi decoction to establish the PD model with the syndrome of liver-yang hyperactivity.Three doses of ZGXF decoction or selegiline were given by a 28-day intragastric administration.The rats were tested for amphetamine-induced rotation asymmetry.In addition,real-time PCR were adopted to analyze the expressions of Nfe212 and Hmox1 mRNAs,while western blot to analyze the expression of Keap1 protein.As a result,it was found that ZGXF decoction dose-dependently attenuated amphetamine-induced rotation,up-regulated the expressions of Nfe212 and Hmox-1 mRNAs,and down-regulated the expression of Keap1 protein in the substantia nigra in PD rats with syndrome of liver-yang hyperactivity.It was suggested that anti-PD effects of ZGXF decoction be attributed to the up-regulation of Nfe212 and Hmox-1 mRNAs and the down-regulation of Keap1 protein,being associated with oxidative stress,in the substantia nigra of PD rats with syndrome of liver-yang hyperactivity.
