Role of oxidation reaction in decrease in activity of acetylcholinesterase in diaphragm of rats with sepsis
10.3760/cma.j.issn.0254-1416.2017.02.020
- VernacularTitle:氧化反应在脓毒症大鼠膈肌乙酰胆碱酯酶活性降低中的作用
- Author:
Jin WU
;
Hua LIU
;
Tian JIN
;
Hong WANG
;
Lianhua CHEN
;
Shitong LI
- Keywords:
Oxidation-reduction;
Sepsis;
Acetylcholinesterase;
Diaphragm
- From:
Chinese Journal of Anesthesiology
2017;37(2):202-204
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of oxidation reaction in the decrease in the activity of acetylcholinesterase (AChE) in the diaphragm of rats with sepsis.Methods Thirty pathogen-free healthy adult male Sprague-Dawley rats,weighing 220-260 g,were divided into 3 groups using a random number table:sham operation group (group S,n=8),sepsis group (group Sep,n =12) and antioxidant group (group Ant,n=10).Sepsis was induced by cecal ligation and puncture.Group S underwent simple laparotomy.N-acetylcystein 20 mg/kg was injected subcutaneously at 3,6,12 and 18 h after operation,and deferoxamine 20 mg/kg was injected subcutaneously at 3 h after operation in group Ant,while the equal volume of normal saline was given instead in S and Sep groups.At 24 h after operation,the surviving rats were sacrificed,and the diaphragm was removed for determination of the malondialdehyde (MDA) content and activities of AChE,myeloperoxidase (MPO),superoxide dismutase (SOD) and catalase (CAT).SOD/CAT ratio was calculated.Results Compared with group S,the AChE activity was significantly decreased,and MPO and SOD activities were increased in Sep and Ant groups,the MDA content and SOD/CAT ratio were significantly increased,and the CAT activity was decreased in group Sep,and the CAT activity was significantly increased in group Ant (P＜0.05).Compared with group Sep,the AChE and CAT activities were significantly increased,and the MDA content,MPO and SOD activities and SOD/CAT ratio were decreased in group Ant (P＜0.05).Conclusion Oxidation reaction is partially involved in the decrease in the activity of AChE in the diaphragm of rats with sepsis.
