Effect of inhaling hydrogen on proteomics during acute lung injury in septic mice
10.3760/cma.j.issn.0254-1416.2017.02.024
- VernacularTitle:吸入氢气对脓毒症小鼠急性肺损伤时蛋白质组学的影响
- Author:
Yingxue BIAN
;
Chao QIN
;
Keliang XIE
;
Yang YU
;
Hongguang CHEN
;
Yanyan WANG
;
Aili DONG
;
Peng ZHANG
;
Yonghao YU
- Keywords:
Hydrogen;
Sepsis;
Respiratory distress syndrome,adult;
Proteomics
- From:
Chinese Journal of Anesthesiology
2017;37(2):218-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of inhaling hydrogen (H2) on proteomics during acute lung injury in septic mice.Methods Sixty male ICR mice,aged 6 weeks,weighing 20-25 g,were divided into 4 groups (n=15 each) using a random number table:sham operation group (Sh group),sham operation plus H2 group (Sh+H2 group),sepsis group (S group) and sepsis plus H2 group (S+H2 group).Sepsis was produced by cecal ligation and puncture.The mice in Sh+H2 and S+H2 groups inhaled 2％ H2 for 1 h starting from 1 and 6 h after operation.At 24 h after operation,lungs were removed for identification of proteins by isobaric tags for relative and absolute quantification and liquid chromatography-tandem mass spectrometry analysis,and the differentially expressed proteins were screened.The differentially expressed proteins were used for KEGG pathway enrichment analysis and STRING protein-protein interaction networks analysis.Western blot was used to confirm the 4 differentially expressed proteins semaphorin 7A,transferrin,OTULIN and mitogen-activated protein kinase kinase kinase 1.Results A total of 4 472 quantifiable proteins were identified.A total of 192 proteins which were related to acute lung injury during H2 inhalationinduced reduction of sepsis were identified.The 192 proteins involved phosphatidylinositol 3-kinase/serine0threonine kinase signaling pathway,chemokine signaling pathway,hypoxia-inducible factor 1 signaling pathway,complement and coagulation cascades,peroxisome proliferator-activated receptor signaling pathway and proteins including ribosome proteins,myosin and troponin,collagen and adhesion-related proteins,coagulation-related proteins found in STRING protein-protein interaction networks.Conclusion Inhaling H2 can induce changes in the expression of 192 proteins,which may be the mechanism of lung protection in septic mice.
