Effects of carboxyamidotriazole on the activation and degranulation of RBL-2H3 cells
- VernacularTitle:羧胺三唑对RBL-2H3细胞活化脱颗粒的影响
- Author:
Yufeng WANG
;
Xiaojuan ZHANG
;
Zehao GUO
;
Juan LI
;
Lei GUO
;
Lei ZHU
;
Caiying YE
- Keywords:
carboxyamidotriazole;
RBL-2H3 cells;
C48/80;
degranulation
- From:
Basic & Clinical Medicine
2017;37(4):479-483
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the anti-infection mechanism of carboxyamidotriazole (CAI) through studying the effects of CAI on the proliferation, apoptosis and degranulation of RBL-2H3 mass cells.Methods Compound 48/80 (C48/80) was used to induce the model of activation and degranulation in RBL-2H3 cells.The morphological change of cell degranulation was observed by neutral red staining.The release levels of histamine and β-hexosaminidase were measured by ELISA method and chromogenic assay, respectively.The cell activity was determined by CCK-8 method.And cell apoptosis was detected by Hoechst 33342 fluorescent staining.Results Compared with the control group, 10, 20, 40 μmol/L CAI inhibited C48/80-induced degranulation of RBL-2H3 cells in different degrees.CAI (20, 40 μmol/L) reduced the histamine release (P<0.01), and CAI (40 μmol/L) decreased the β-hexosaminidase release (P<0.01).In addition, the viability and apoptosis of RBL-2H3 cells were not affected at the concentrations of CAI used.Conclusions CAI can effectively inhibit the activation and degranulation of RBL-2H3 mast cells, and this effect is not through cytotoxicity.The anti-infection effect of CAI may partially due to the down-regulation of mast cell activity.
