Investigation of Microglia Activation and Inflammatory Cytokine Changes in Experimental Rabbits After Spinal Cord Ischemia Reperfusion
10.3969/j.issn.1000-3614.2017.04.020
- VernacularTitle:兔脊髓缺血再灌注后小胶质细胞活化及炎性细胞因子变化的研究
- Author:
Yunlu WANG
;
Lei TIAN
;
Shiyao LIU
;
Zhigao MA
;
Siyu HOU
;
Yanwei YANG
;
Huixian LI
;
Mu JIN
;
Xiuhua DONG
;
Jiakai LU
;
Weiping CHENG
- Keywords:
Reperfusion injury;
Microglia cell;
Inflammatory Chemokine
- From:
Chinese Circulation Journal
2017;32(4):395-400
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the activation of microglia and the changing rule of inflammatory cytokine as IL-6, IL-10 and nuclear factor-κB (NF-κB) in experimental rabbits after spinal cord ischemia reperfusion (SCIR) injury in order to provide theoretical basis for post-conditioning time. Methods: Rabbit SCIR injury model was established by thoracic aorta balloon occlusion. 54 New Zealand male adult white rabbits were divided into 9 groups: Sham group (the animals received balloon implantation without occlusion), SCIR-0h group (reperfusion was conducted at 0 hour of spinal cord ischemia), SCIR-1h, -2h, -3h, -8h, -24h,-48h and -72h groups. n=6 in each group. The number of normal and apoptosis neurons, the levels of Iba-1, IL-6, IL-10 and NF-κB in spinal tissue were examined and compared among different groups respectively. Results: The number of normal neuron was decreasing with the extended reperfusion time, TUNEL-positive neuron began to increasing in SCIR-8h group and the peak was reached in SCIR-24h group. The expression of Iba-1 began to elevating in SCIR-2h group and the peak was obtained in SCIR-8h group; NF-κB began to rising in SCIR-3h group and the peak was observed in SCIR-8h group; both IL-6 and IL-10 arrived the peak in SCIR-24h group. The expressions of NF-κB, IL-6 and IL-10 were positively related to Iba-1 level. Conclusion: Microglia activation had dynamic changes in experimental SCIR rabbits and the expression levels of NF-κB, IL-6 and IL-10 were positively to microglia activation; post-conditioning time at front and back to microglia activation may reduce neuron injury.
