Characteristics and in vitro cell compatibility of human acellular dermal matrix with improved method
10.3760/cma.j.issn.1671-0290.2016.06.015
- VernacularTitle:改良法制备人脱细胞真皮的特性与体外细胞相容性
- Author:
Tao JIANG
;
Peisheng JIN
;
Changbo TAO
;
Yanping GUO
;
Linxia ZHANG
;
Yu WANG
;
Aijun ZHANG
- Keywords:
Acellular dermal matrix;
Cell infiltration;
Tissue engineering skin
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2016;22(6):369-372
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the characteristics and in vitro cell compatibility of human acellular dermal matrix (ADM) with the improved method.Methods Cell components of healthy human skins were removed by the improved method and the traditional method respectively.The porosity, degradation time in vitro of the ADM prepared by two methods and the cytotoxicity of the material infiltration liquid with the improved method on the adipose derived stem cells were detected.HE staining was used to detect the residual of the cells, the integrity of collagen and cell biocompatibility.Scanning electron microscopy (SEM) was used to detect the pore size.Results Both the two methods could completely remove the cells, and maintain the integrity of the collagen scaffold;The porosity of ADM with the improved method was higher (93.1±1.02)% than that of traditional method (74.27±2.04)% (P<0.05);There was no significant difference in the cytotoxicity and in vitro degradation time between the two kinds of ADM;While pore diameter of the improved method was significantly higher [(181.21±66.9) μm] than that [(102.38±15.63) μm] in dermal reticular surface with the traditonal method (P<0.05).Conclusions There is no obvious cytotoxicity of the ADM with the improved method, and therefore it is more suitable for cell adhesion growth with higher porosity and larger pore size.
