Down-regulation of miR-31-5p in human acute myeloid leukemia
- VernacularTitle:miR-31-5p在急性髓系白血病中的表达下调
- Author:
Lixia CAO
;
Lianxiang CHEN
- Keywords:
miR-31-5p;
THP-1;
HuR;
acute myeloid leukemia
- From:
Basic & Clinical Medicine
2017;37(4):537-542
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression and roles of miR-31-5p in acute myeloid leukemia (AML).Methods miR-31-5p in AML patients were evaluated by real-time PCR;THP-1 cells were transfected with the miR-31-5p mimic and control, respectively.The effects of over-expression of miR-31-5p were examined by CCK-8 and FACS analysis;Dual-luciferase and Western blot were performed to detect target gene HuR expression.The effects of knock-down of HUR were also examined by CCK-8 and FACS analysis.Results miR-31-5p was down-regulated in AML patients compared to the normal control.Over-expression of miR-31-5p in THP-1 cells reduces cell proliferation and accelerates monocytic differentiation.miR-31-5p could target HuR, and knock-down of HuR inhibits cell proliferation and attenuates monocytic differentiation in THP-1 cells.Conclusions miR-31-5p may regulate AML cell proliferation and monocytic differentiation by targeting HuR.
