Preliminary mechanism study of HCoV-OC43 escape from human dendritic cell immune elimination
10.3969/j.issn.1000-484X.2017.04.002
- VernacularTitle:HCoV-OC43逃避人树突状细胞免疫清除机制的初步研究
- Author:
Quan YANG
;
Jiuling TUO
;
Xubin HUANG
;
Hongjiao LUO
;
Kai ZHOU
;
Tian ZHANG
;
Kaiyuan CAO
;
Lin XU
- Keywords:
Human coronavirus OC43;
Human dendritic cells;
Cytokine;
Immune escape
- From:
Chinese Journal of Immunology
2017;33(4):488-493
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the possible immune escape mechanisms of HCoV-OC43 from human dendritic cells(DC).Methods:HCoV-OC43 was isolated from clinical specimen using BSC-1 cells and identified by Real-time PCR,and the cytopathic effect was observed by phase contrast microscope.DCs were induced in vivo using hu-GM-CSF and IL-4 cytokines,and after 7 days of differentiation,DCs were infected by HCoV-OC43.The morphology of HCoV-OC43 infected DC was observed by transmission electron microscope,and the cytokines related to DC functions were detected by Real-time PCR after infection.DC proportion and function related co-stimulatory molecules were analyzed by flow cytometry.Results:In vitro HCoV-OC43 infected human DC model was successfully built.HCoV-OC43 can infect DC and generate immune response of DC in vitro,but no virus nucleonic acid could be detected in culture supernatant.The DC expression of IFN-α,IFN-β,CCL3 and CCL5 were significant decreased when infected with HCoV-OC43,but the expression of costimulatory molecules including HLA-DR,CD1c and CD86 were not affected by HCoV-OC43 infection.Conclusion:Human DC could be infected by HCoV-OC43 and generate immune response,but could not produce progeny virus.HCoV-OC43 may escape from immune response by suppressing the expression of IFN-α and other inflammatory cytokines and chemokines in DC.
