Interferon alpha suppresses proliferation and induces apoptosis of human brain vascular adventitial fibroblasts via IFI16
10.3969/j.issn.1000-484X.2017.04.003
- VernacularTitle:IFN-α通过IFI16抑制人脑血管外膜成纤维细胞增殖并促进细胞凋亡
- Author:
Jing HUANG
;
Fang SONG
;
Xiangshu LONG
;
Maobo TIAN
;
Qiang WU
- Keywords:
Interferon-inducible protein;
IFN-α;
Vascular adventitial fibroblasts;
Proliferation;
Apoptosis
- From:
Chinese Journal of Immunology
2017;33(4):494-497
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study interferon alpha (IFN-α) inhibition of proliferation and apoptosis induction of human brain vascular adventitial fibroblasts(HBVAFs)via IFI16.Methods:Cultured HBVAFs were treated with transfection IFI16 siRNA and/or IFN-α in vitro instantaneously.The protein and mRNA levels of IFI16,P53,P21 were measured by Western blot and Real-time PCR.MTT was used to detect the cell proliferation of the HBVAFs.Cell cycle and apoptosis were analyzed by flow cytometry.Results:IFN-α with terminal concentration of 2 000-5 000 kU/L induce significantly expression of IFI16 in HBVAFs,without any significant difference.Stimulated with 2 000 kU/L IFN-α up-regulated the expression of P53,P21 at protein and mRNA levels,and inhibited the cell proliferation and promote cells apoptosis in HBVAFs.Such effect was restrained by transfection with IFI16 siRNA into HBVAFs.Conclusion:IFN-α inhibits HBVAFs proliferation and induces apoptosis may partly relate to the increased IFI16 expression.
