IL-22 inhibits liver fibrosis induced by hepatic stellate cells via Wnt/β-catenin signal pathway
10.3969/j.issn.1000-484X.2017.04.005
- VernacularTitle:IL-22经Wnt/β-catenin通路抑制肝星状细胞致纤维化作用
- Author:
Cheng SHI
;
Ronge LEI
;
Bangli HU
;
Peiling ZHANG
;
Shanyu QIN
;
Haixing JIANG
- Keywords:
Hepatic stellate cells;
Interleukin-22;
Wnt/β-catenin signal pathway
- From:
Chinese Journal of Immunology
2017;33(4):502-506
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects and mechanisms of interleukin-22(IL-22) on inhibiting liver fibrosis induced by HSC,and explore the role of Wnt/β-catenin pathway in the activation of hepatic stellate cells(HSC).Methods:Rat HSC was activated by TGF-β1,and the mRNA and protein levels of β-catenin and α-SMA were detected by q-PCR and Western blot,respectively.HSC was treated with different hours and concentration of recombinant rat protein IL-22.The cell proliferation rates were detected by CCK8,cell apoptosis rates were tested by flow cytometry.HSC were treated with optimal concentration of IL-22 after activated by TGF-β1,the cell proliferation rates,mRNA and protein levels of β-catenin and α-SMA were compared of before and after intervention.Results:The mRNA and the protein levels of β-catenin and α-SMA were significantly increased after activated by TGF-β1(P<0.05).IL-22 inhibiting the proliferation of HSC in a dose-and time-dependent manner (P<0.05) and decreased the mRNA and the protein expression level of β-catenin and α-SMA(P<0.05),but had no significant effect on apoptosis rates(P>0.05).IL-22 significantly inhibited the activation of HSC induced by TGF-β1 and remarkably decreased the mRNA and the protein expression level of β-catenin and α-SMA(P<0.05).Conclusion:The Wnt/β-catenin pathway may participates in the process of HSC activation and α-SMA secretion,and IL-22 inhibits biological function of HSC in a dose-and time-dependent manner.This effect probably via inhibited the Wnt/β-catenin signal pathway.
