The expression and clinical significance of miR-98 in peripheral blood mononuclear cells in asthmatic children
10.3760/cma.j.issn.1673-4912.2017.03.009
- VernacularTitle:miR-98在哮喘患儿外周血单个核细胞中的表达及意义
- Author:
Xueqing XIAO
;
Wanhong XIA
;
Hong ZHAI
;
Jun PENG
- Keywords:
Asthma;
MiR-98;
Interleukin-4;
Interleukin-13
- From:
Chinese Pediatric Emergency Medicine
2017;24(3):201-204
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of miR-98 in peripheral blood mononuclear cells (PBMC) in the pathogenesis and development of childhood asthma.Methods A total of 43 cases of asthmatic children and 30 cases of healthy controls were enrolled in the study.Peripheral blood mononuclear cells were isolated in both healthy subjects and asthmatic children in acute attack and remission stages.The expressions of miR-98 and interleukin-4(IL-4) and IL-13 mRNA were detected by real-time quantitative PCR.Results The miR-98 levels of asthmatic children in attack stage were significantly lower than those in remission stage and control group (P<0.01).The IL-4 and IL-13 mRNA levels of asthmatic children in attack stage were significantly higher than those in remission stage and control group (P<0.01).There was no significant difference of miR-98,IL-4 and IL-13 mRNA between asthmatic children in remission stage and the controls (P>0.05).Furthermore,a negative correlation was found between the expression of miR-98 and IL-5(r=-0.794,P<0.01) and between the expression of miR-98 and IL-13 mRNA (r=-0.804,P<0.01) in asthmatic children in attack stage.A positive correlation was also found between IL-4 and IL-13 mRNA in asthmatic children in attack stage (r=0.853,P<0.01).Conclusion The expression of miR-98 decreased in asthmatic children,and miR-98 might be involved in the pathogenesis and development of asthma.
