Effect of Fuzheng Jiedu Tongluofang on MMP-2 and MMP-9 and invasion of human hepatoma HepG2 cells
10.3969/j.issn.1000-484X.2017.04.013
- VernacularTitle:扶正解毒通络方对人肝癌HepG2细胞MMP-2、MMP-9表达及细胞侵袭作用的影响
- Author:
Jingzhou ZHANG
;
Xiangwei FENG
;
Fanping MENG
- Keywords:
Fuzheng Jiedu Tongluofang;
Hepatioma HepG2 cells;
MMP-2;
MMP-9;
Invasion
- From:
Chinese Journal of Immunology
2017;33(4):542-544
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of Fuzheng Jiedu Tongluofang on the expression of MMP-2,MMP-9 and invasion of human hepatoma HepG2 cell.Methods:Hepatioma HepG2 cell was treated with Fuzheng Jiedu Tongluofang.The cell viability was measured by CCK8.The invasion of HepG2 was detected by Transwell assay.The expression of MMP-2 and MMP-9 was analyzed by ELISA.Results:In the CCK8 assay,Fuzheng Jiedu Tongluofang could inhibit the proliferation of HepG2 cells at a density and time measure.In the Transwell assay,the inhibitory rate of invasion was 52.45% when treated with Fuzheng Jiedu Tongluofang at a concentration of 2.65 mg/ml. The ELISA assay indicated that the expression of MMP-2 and MMP-9 decreased significantly after treated with Fuzheng Jiedu Tongluofang(P<0.05). Conclusion:Fuzheng Jiedu Tongluofang suppressed the invasion of HepG2 with the possible mechanism of down-regulating the expression of MMP-2 and MMP-9 in HepG2.
