Study on the role of bone morphogenetic protein 4 in mediating acid and glycocholic acid induced Barrett′s esophagus
10.3760/cma.j.issn.0254-1432.2017.03.011
- VernacularTitle:骨形成蛋白4介导的酸和甘氨胆酸诱导Barrett食管的作用研究
- Author:
Baoru DENG
;
Yangyang HUI
;
Lanping ZHU
;
Junyi GUO
;
Shuling WANG
;
Ningning ZHAO
;
Xin CHEN
;
Bangmao WANG
- Keywords:
Barrett esophagus;
Bone morphogenetic protein 4;
Caudal-related homeobox transcription factor 2;
Fibroblasts;
Human esophageal epithelial cell
- From:
Chinese Journal of Digestion
2017;37(3):179-182
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of bone morphogenetic protein 4(BMP4) in the genesis of Barrett′s esophagus.Methods Human esophageal epithelial cell (HEEC) and MRC-5 were cultured.The effects of different concentration of BMP4 and different pH value of hydrochloric acid or glycocholic acid on the expression of caudal-related homeobox transcription factor 2(CDX2) in HEEC were detected by real time polymerase chain reaction.The effects of different pH value of hydrochloric acid or glycocholic acid on BMP4 expression in MRC-5 were also investigated.Independent sample t test was performed for statistical analysis.Results After HEEC stimulated by BMP4 at 0.1, 1.0, 10.0 and 100.0 ng/mL, the relative quantity expressions of CDX2 were 1.617±0.246, 2.489±0.455, 5.629±0.449 and 13.670±1.689, respectively, which were higher than those of control group (1.000±0.043, 1.029±0.094, 1.001±0.002 and 1.049±0.051, respectively), and the differences were statistically significant (t=2.47, 3.14, 10.31, 7.47;all P<0.05).After MRC-5 stimulated by acid at pH four or five, or glycocholic acid at pH four or five, the relative quantity expressions of BMP4 were 2.430±0.105, 2.394±0.145, 125.900±12.620 and 2.128±0.215, respectively, which were higher than those of control group(1.025±0.095, 0.999±0.007, 1.060±0.138 and 0.893±0.110,respectively), and the differences were statistically significant (t=9.94, 9.59, 9.89, 5.11;all P<0.01).Conclusion BMP4 can increase the expression of CDX2 in HEEC, which promote the genesis of Barrett′s esophagus.
