Ultra Performance Liquid Chromatography-Tandem Mass Spectrometric Method for Detection of Pork Peptide Biomarkers
10.11895/j.issn.0253-3820.160491
- VernacularTitle:超高效液相色谱-串联质谱法鉴定猪肉的特异性肽生物标志物
- Author:
Guangyun ZHOU
;
Guiji WANG
;
Haowei REN
;
Qian LU
;
Yan YANG
;
Lihai GUO
;
Ning LIU
- Keywords:
Pork;
Ultra performance liquid chromatography-tandem mass spectrometry;
Multiple reaction monitoring;
Peptide biomarkers
- From:
Chinese Journal of Analytical Chemistry
2017;45(2):205-210
- CountryChina
- Language:Chinese
-
Abstract:
The samples of muscular tissue from pork,beef and lamb which were closely related in the genetic relationship were analyzed by ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS) technique.The specific peptide biomarkers of pig meat species were found and confirmed.Proteins from three pure meat samples were extracted and digested using trypsin,the digested proteins were identified by UPLC-triple time-of-flight (TOF)-MS,and the total ion chromatogram (TIC) was searched and analyzed against the UniProt database.Three high abundant homologous proteins of three species and 8 potential peptide biomarkers of pork were found.A multiple reaction monitoring (MRM) QTRAP-MS method was established to confirm the specificity of potential peptide biomarkers.As a result,five peptide biomarkers of pig species meat were confirmed,three of which were not reported.
