Peptidome Analysis of Transudative Pleural Effusion by Ultra-filtration Coupled with Nano-Liquid Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.160751
- VernacularTitle:超滤法结合纳升液相色谱-串联质谱技术分析漏出性胸腔积液中多肽组分
- Author:
Shifan CHEN
;
Jingbo YANG
;
Min ZHANG
;
Xiaoou LI
;
Xiaofeng LIU
;
Honghua GUO
;
Chengyan HE
;
Hongwen GAO
- Keywords:
Peptidome;
Ultrafiltration;
Nano-liquid chromatography-tandem mass spectrometry;
Transudative pleural effusion
- From:
Chinese Journal of Analytical Chemistry
2017;45(2):224-230
- CountryChina
- Language:Chinese
-
Abstract:
The peptides,proteins and other biological molecules in transudative pleural effusion correlate directly or indirectly with specific physiological and pathological state,reflecting the information regarding the lungs or other parts of the body.In the present study,the peptide fraction in transudative pleural effusion was isolated by uhrafiltration.After desalted and enriched by C18 tips,the peptide mixture was analyzed by nano LC-MS/MS.The results showed that 314 peptides,which were originated from 52 proteins,in pleural transudate were identified.More than half of the peptides were derived from fibrinogen.Many peptides were characterized as displaying ladder sequences.In addition,a large number of proline oxidation modifications were detected in the peptides derived from collagen and fibrinogen.Gene ontology enrichment analysis showed that the most of the proteins extracellular properties of pleural transudate polypeptide components were protein with exocytosis.The study provided a rapid and efficient separation and analysis methods for lung disease markers related peptide compounds in pleural fluid leakage.Also this research provided a rapid and effective method for screening peptide biomarkers related to lung diseases from transudative pleural effusion.
