Screening and Structure Characterization of Acetylcholinesterase Inhibitors from Total Alkaloids of Fibraurea recisa Pierre.by Target Molecule Affinity-Liquid Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.160460
- VernacularTitle:靶向亲和-液相色谱-质谱联用技术快速筛选黄藤总生物碱中乙酰胆碱酯酶抑制剂
- Author:
Zhongmei HE
;
Na LYU
;
Minlun NAN
;
Yuwei ZHAO
;
Yufang HE
;
Lingwen MENG
;
Jiaming SUN
;
Lianxue ZHANG
- Keywords:
Target molecule affinity-liquid chromatography-tandem mass spectrometry;
Total alkaloids of Fibraurea recisa Pierre.;
Acetylcholinesterase;
Inhibitor
- From:
Chinese Journal of Analytical Chemistry
2017;45(2):211-216
- CountryChina
- Language:Chinese
-
Abstract:
A target molecule affinity-ultrafiltration liquid chromatography-electrospray ionization tandem mass spectrometric (LC-ESI-MSn) method was established for rapid screening acetylcholinesterase (AChE) inhibitors from total alkaloids of fibraurea recisa Pierre.A total of 12 potential inhibitors were screened from Fibraurea recisa Pierre.and 6 compounds were identified including palmatine,berberine,jatrorrhizine,palmatrubine,7,8-dihydro-8-hydroxyberberine and groenlandicine.The AChE inhibitory activity of these 6 compounds was validated in vitro.Palmatine showed the strongest inhibitory activity for AChE,which was stronger than that of donepezil hydrochloride,demonstrating the potential of palmatine as anti-Alzheimer's drug.This method is simple,rapid,and accurate for directly screening active ingredients which can inhibit AChE from complex extract of traditional Chinese medicines.
