Effect and Underlying Mechanism of Liraglutide on Nitrogen Monoxide Release in Human Umbilical Vein Endothelial Cells in the State of Hypoxia and High Glucose
- VernacularTitle:利拉鲁肽对缺氧和高糖状态下人脐静脉内皮细胞释放NO的影响及机制研究
- Author:
Yinke LI
;
Chen CHEN
;
Qiongzhi SHI
;
Xiangru LIAO
;
Xiangyang XIE
- Keywords:
Glucagon like peptide-1;
Liraglutide;
Endothelial cells;
NO;
eNOS
- From:
China Pharmacist
2017;20(4):652-655
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects and mechanism of liraglutide on nitrogen monoxide (NO) release in human umbilical vein endothelial cells in the state of hypoxia and high glucose.Methods:A model of hypoxia and high glucose was established by using isolation and culture of primary human umbilical vein endothelial cells (HUVECs) in vitro.HUVECs were incubated with liraglutide and/or exendin (9-39) for 4 h.The metabolic ability of cells was detected by MTT assay,the activity of lactate dehydrogenase (LDH) was measured by a colorimetric method,and the levels of extracellular NO were measured by a nitrate reductive enzymatic method.The endothelial nitric oxide synthase (eNOS) mRNA expression was detected by reverse transcription-polymerase chain reaction (RT-PCR).Results:Compared with the model group,liraglutide could significantly increase cell metabolic ability,reduce LDH release,increase NO release and eNOS mRNA expression (P<0.05 or P<0.01).The above effects of liraglutide were partly inhibited by glucagon like peptide-1 (GLP-1) receptor antagonist exendin (9-39)(P<0.05).Conclusion:Liraglutide can improve endothelial relaxation function in HUVECs in the state of hypoxia and high glucose in vitro.The effect might be related to up-regulating eNOS mRNA expression and promoting NO release.
