Autophagy protects against histones-mediated extracellular apoptosis in proximal tubules
10.3969/j.issn.1006-5725.2017.03.005
- VernacularTitle:自噬减少组蛋白介导的肾小管上皮细胞凋亡
- Author:
Xiaoli HONG
;
Yangyang WANG
;
Shaoye CUI
;
Ping CHANG
- Keywords:
Autophagy;
Histone;
H K-2 apoptosis
- From:
The Journal of Practical Medicine
2017;33(3):350-353
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of autophagy on histone-mediated apoptosis of human proximal tubular endothelial cells(HK-2).Methods To investigate the effect of histones on the autophagy and apoptosis,HK-2 cells were treated with increasing concentrations of histones.The rate of apoptosis and the expressions of autophagy-related protien LC3Ⅱ and Beclin1 in HK-2 cells were detected by using flow cytometry and immunoblotting assay,respectively.To further confirm the effect of autophagy on apoptosis of HK-2 cells,cells were incubated with histones after one hour pretreatment with 10 mmol/L 3-MA,a pharmacological inhibitor.The rate of apoptosis and the activity of caspase-3 of HK-2 cells were detected separately by using flow cytometry and immunoblotting assay.Results Histones significantly enhanced apoptosis of HK-2 cells in a dose-dependent manner,with the increased expressions of LC3Ⅱ and Beclin1.Blockage of autophagy by 3-MA significantly increased the apoptosis of HK-2 cells and the activity of caspase3.Conclusion Autophagy in proximal tubules protects against apoptosis induced by histones,with potential value in acute kidney injury (AKI).
