Advance and application of CRISPR/ Cas9 mediated genome editing technique on pathogenic microorganism
10.3969/j.issn.1002-2694.2017.03.017
- VernacularTitle:CRISPR/Cas9基因编辑技术在病原微生物中的应用
- Author:
Dingjie AN
;
Yuanhuan KANG
;
Long CHEN
;
Haiyue ZHANG
;
Dongxing ZHANG
;
Jiaxin TIAN
;
Junpeng JIA
;
Wuwen SUN
;
Xiaofeng SHAN
;
Aidong QIAN
- Keywords:
geneome editing;
CRISPR/Cas9;
pathogenic microorgaisms;
off-target effect
- From:
Chinese Journal of Zoonoses
2017;33(3):280-286
- CountryChina
- Language:Chinese
-
Abstract:
Clustered regularly interspaced short palindromic repeats (CRISPR) is an acquired immune system existing in archaea and bacteria with the long-term process of evolutionary.CRISPR/Cas9 gene editing system is a new type of gene editing technology developed based on the system.CRISPR/Cas9 is a more efficient method for gene targeting than the previous methods.It has been successfully applied for gene-modified of eukaryotes since 2012,but the reports about pathogenic microorgaisms are rarely.Here,the research progress in the structure,mechanism of CRISPR/Cas9 system and its applications on pathogenic microorgaisms is reviewed.
