A Quality Comparison Between the Precise Powder Decoction Pieces and the Original Slices of Spatholobus suberectus Dunn.
10.11842/wst.2017.01.012
- VernacularTitle:鸡血藤精准煮散饮片与原饮片质量的对比性研究
- Author:
Wen XU
;
Di LEI
;
Jing ZHANG
;
Jiang XU
;
Zhihai HUANG
;
Xiaohui QIU
;
A.hawkins JULIE
;
Shilin CHEN
- Keywords:
Precise powder decoction pieces;
Spatholobus suberectus dunn;
uniformity;
DNA barcode;
quality system
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2017;19(1):83-88
- CountryChina
- Language:Chinese
-
Abstract:
In this study,DNA molecular identification technology and chemical fingerprint method were adopted to evaluate the quality system of precise powder decoction pieces (PPDP) of S.suberectus dunn (SSD).ITS2 sequence was taken as DNA barcode to indentify SSD.Different specifications of PPDP were prepared,their dry extract contents were quantified in contrast with that of original slices.Three batches of SSD original slices were gleaned and the content uniformity,fingerprint and similarity evaluation before and after the mixing and pulverization were valued by HPLC-DAD.As a result,ITS2 successfully and accurately identified the SSD in this study.The extract rate of PPDP was 15.5％,1.11 times as much as the original slices.RSD of inter-assay dissolution of cepicatechin from the original slices was 11.0％,which was reduced to 1.0％ after mixing and preparing into PPDP.The relative peak area of the 14 common peaks identified by fingerpringts were larger,while the RSD values significantly decreased.It was concluded that the PPDP of SSD improved the extraction efficiency and uniformity of the original slices,featuring quite prospective in more reasonable and scientific clinical use.
