A Research on the Anti-tumor Effects of Acid-Soluble Polysaccharides from Gloeostereum incamatum in H22 Tumor Bearing Mice
10.11842/wst.2017.01.021
- VernacularTitle:榆耳酸溶性多糖对肝癌H22小鼠的抑瘤作用研究
- Author:
Hong WANG
;
Yan ZHAO
;
Enbo CAI
;
Tolgor BAU
;
Wei LI
;
Yu LI
;
Haiying BAO
- Keywords:
Acid-soluble polysaccharides of Gloeostereum incamatum;
H22 tumor bearing mice;
anti-tumor;
H&E staining;
TUNEL
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2017;19(1):142-148
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed at exploring the inhibitory effect behind its mechanism on acid-soluble polysaccharides from G.incamatum in transplanted H22 tumor mice.Different indices,including tumor inhibitory rate,organ index of liver,thymus and spleen,IL-2,IFN-γ and TNF-α were detected for the evaluation of anti-tumor effects and the mechanism.Furthermore,HE staining and TUNEL assay were adopted to investigate the pathological changes of tumor tissue and cell apoptosis,respectively.As a result,the three dose groups of acidsoluble polysaccharides of G.incamatum successfully inhibited the proliferation of tumor cells,while organ indexes of spleen and thymus were improved and serum IL-2,IFN-γ and TNF-α increased.H&E staining and TUNEL assay showed the polysaccharides induced cell apoptosis,playing a significant role in the inhibition of tumor growth.In conclusion,acid-soluble polysaccharides of G.incamatum possessed significant anti-tumor effects,behind which the mechanism could be related to the regulation of immune regulation,cell apoptosis,and the protection of liver function.
