Preconditioning of ulinastatin alleviates GES-1 cell injury induced by oxygen and glucose deprivation
10.3969/j.issn.1006-5725.2017.06.003
- VernacularTitle:乌司他丁预处理可减轻氧糖剥夺诱导的GES-1细胞损伤
- Author:
Yao WANG
;
Wenbin XI
;
Youping WU
;
Ji JIA
;
Weifeng TU
- Keywords:
Ulinastatin;
Preconditioning;
GES-1 cells;
Oxygen and glucose deprivation
- From:
The Journal of Practical Medicine
2017;33(6):858-862
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of the preconditioning of ulinastatin on GES-1 cell injury induced by oxygen and glucose deprivation (OGD). Methods GES-1 cells were cultured in vitro and divided into three groups: normal control group (group N), oxygen and glucose deprivation group (group O), and ulinastatin preconditioning group (group U). The OGD model of GES-1 cells were established by glucose-free medium and three-gas incubator for 6h. Ulinastatin was added to group U 12h before the deprivation of oxygen and glucose. The cell viability and apoptosis were determined by cck-8 and flow cytometry respectively. Western Blot was used to examine the protein expression of Caspase-3 and Cleaved Caspase-3. The TRPV1 mRNA expression was measured by quantitative real-time PCR. Results As compared with group N, the viability of GES-1 was decreased, the apoptotic rate and the expression of Caspase-3 and Cleaved Caspase-3 were increased, and the TRPV1 mRNA expression decreased greatly in group O (P < 0.05). As compared with group O, the aforementioned changes were significantly inhibited in group U. Conclusions Ulinastatin preconditioning could effectively inhibit GES-1 cell injury induced by OGD, which may be related to the inhibition of apoptosis and the upregulation of TRPV1 mRNA expression.
