Effect of endothelial colony-forming cells conditioned medium on biological function of human dermal fibroblasts
10.3969/j.issn.1006-5725.2017.06.004
- VernacularTitle:内皮克隆形成细胞条件培养基对人真皮成纤维细胞生物学作用的影响
- Author:
Bulin WANG
;
Yanhong WU
;
Yuzhi WANG
;
Xiaorui GUO
;
Qin LI
- Keywords:
Endothelial progenitor cells;
Endothelial colony-forming cells;
Conditioned medium;
Fibroblasts;
Wound healing
- From:
The Journal of Practical Medicine
2017;33(6):862-866
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the effect of endothelial colony-forming cells conditioned medium(ECFCs-CM)on biological function of human dermal fibroblasts(HDFs). Methods Human cord blood derived-ECFCs were isolated and identified based on the previous studies. The cytokines in ECFCs-CM were detected using a cytokines antibody array. HDFs were cultured with ECFCs-CM,using serum free EBM-2 as control. The proliferation of HDFs was examined by Cell Counting Kit-8(CCK-8)and the migration was assessed by scratch test assay. The apoptosis of HDFs was detected by flow cytometry. Results The cells isolated from human cord blood demonstrated typical characteristics of ECFCs. The cytokines antibody array indicated that ECFCs-CM contained large amounts of secreted cytokines such as PDGF-BBand EGF. Compared with the control group,the HDFs cultured with ECFCs-CM showed improved proliferation and migration ability. The number of apoptotic cells was smaller than that of the control group under the environment of serum starvation. Conclusion ECFCs-CM can promote the proliferation and migration of HDFs and inhibit the apoptosis of HDFs under the environment of serum starvation.
