miR-25 promotes gastric cancer cells proliferation by directly targeting KLF4
10.3969/j.issn.1006-5725.2017.06.005
- VernacularTitle:miR-25靶向抑制KLF4促进胃癌细胞增殖
- Author:
Xiaoli DING
;
Yu XIA
;
Rong HU
- Keywords:
Stomach neoplasms miR-25;
KLF4;
ERK Pathway
- From:
The Journal of Practical Medicine
2017;33(6):867-871
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate whether miR-25 targets KLF4 to regulate the growth of gastric cancer cells and lucidate the mechanism of miR-25 producing functional effects in gastric cancer cells. Methods The expression levels of miR-25 and KLF4 were detected by qRT-PCR in 35 pairs of gastric cancer tissues of patients. Target genes of miR-25 were predicted using Targetscan and verified though dual luciferase activity assay and west-ern blot. MTT assay and clone formation assay were detected after downregulated miR-25 and overexpressed KLF4 respectively. Results miR-25 was highly expressed in gastric cancer tissues with KLF4 downexpresstion. Downreg-ulated miR-25 and overexpressed KLF4 respectively can significantly reduce the proliferation of gastric cancer cells,the results were opposite when overexpression of KLF4. Conclusion miR-25 promotes gastric cancer cells proliferation by targeting KLF4.
