Lentiviral vector mediated up?regulation of miR?126 promotes migration and invasion on AGS cells
10.3969/j.issn.1006?5725.2017.05.006
- VernacularTitle:慢病毒载体介导的miR?126稳定高表达促进胃癌AGS细胞的迁移和侵袭
- Author:
Xiaofeng YIN
;
Yue LI
;
Xin LI
;
Haixia LI
;
Yurong QIU
- Keywords:
miR?126;
Metastasis;
Invasion;
Lentivirus;
Stable expression
- From:
The Journal of Practical Medicine
2017;33(5):696-700
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an AGS cell line that stably expressing miR?126 and to study the effect of miR?126 on the proliferation and metastatic abilities of the AGS cell line in vitro. Methods AGS cells were infected by lentivirus with Lv?has?mir?126. After confirmation by RT?PCR ,CCK?8 and clone formation assays were used to evaluate the effect of miR?126 on AGS cell growth. Transwell migration and invasion assays were used to evaluate the effect of miR?126 on metastasis of AGS cells. Results We verified correct construction of recombinant AGS cells. RT?PCR confirmed mRNA levels of miR?126 existed significantly differences among the recombinant cell lines (P< 0.05). Proliferation assays and clone formation assays did not show a remarkable growth suppression in AGS?mir?126 cell line. However,transwell assay showed a notable acceleration in AGS?mir?126(P< 0.05). Conclusions We successfully constructed recombinant AGS cell line with stably high miR?126 expression level. MiR?126 could facilitate the metastasis of AGS cell in vitro.
