A histologic study on osteophytes in severe osteoarthritis by Masson staining and immunohistochemical staining for collagen type Ⅱ
10.3969/j.issn.1006?5725.2017.05.024
- VernacularTitle:利用Masson染色联合Ⅱ型胶原蛋白免疫组化染色对严重骨关节炎骨赘的组织学研究
- Author:
Peng LI
;
Yue WANG
- Keywords:
Osteoarthritis;
Osteophyte;
Histology;
Classification
- From:
The Journal of Practical Medicine
2017;33(5):762-766
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a histological classification system for osteophytes by Masson staining and immunohistochemical staining for collagen type II. Methods Osteophytes were collected from knee joints in os?teoarthritis patients after joint replacement surgery. To develop an osteophyte classification ,serial tissue sections were identified using histological(hematoxylin and eosin,Masson ' s trichrome,toluidine blue)and immunohisto?chemical staining(collagen typeⅡ). Joint function was assessed by WOMAC score and radiological findings was measured by Kellgren?Lawrence grading. The consistency between the osteophyte classification and WOMAC/Kell?gren?Lawrence grading were evaluated. Results 31 patients and 101 osteophyte samples were included in this study. Based on the histological and immunohistochemical evaluation,osteophytes can be divided into type A (4.9%),type B(4.0%),type C(26.7%)and type D(64.4%). This histological classification system had better consistency with joint function score(χ2 = 0.108,P = 0.947)and Kellgren?Lawrence grading(χ2 = 3.205,P =0.201). Conclusions This trial establishes a histological classification system for osteoarthritis osteophytes ,types A and B indicating early osteophyte and types C and D indicating advanced osteophyte. The system is helpful in clini?cal and basic research on osteoarthritis.
